5V6A

Crystal structure of the Middle East respiratory syndrome coronavirus papain-like protease bound to ubiquitin variant ME.2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	93
Detector technology	CCD
Collection date	2015-12-18
Detector	RAYONIX MX300HE
Wavelength(s)	1.2811
Spacegroup name	C 2 2 21
Unit cell lengths	47.071, 109.779, 183.863
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.966 - 2.700
R-factor	0.2222
Rwork	0.217
R-free	0.27300
RMSD bond length	0.001
RMSD bond angle	0.398
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((dev_2481: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.970
High resolution limit [Å]	2.700
Number of reflections	13337
<I/σ(I)>	16.69
Completeness [%]	98.7
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	294.15	0.1 M trisodium citrate pH 5.6, 19% (w/v) PEG 4000, 19% (v/v) 1,2-isopropanediol