5V5X

Protocadherin gammaB7 EC3-6 cis-dimer structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	P 21 21 21
Unit cell lengths	68.242, 91.762, 452.991
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.962 - 3.500
R-factor	0.2103
Rwork	0.208
R-free	0.25670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5szr
RMSD bond length	0.003
RMSD bond angle	0.553
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	3.500
Rmerge	0.161
Number of reflections	36143
<I/σ(I)>	6.8
Completeness [%]	98.0
Redundancy	3.2
CC(1/2)	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7.5	295	20% ethylene glycol, 10% PEG8000, 10% Morpheus amino acids additive (Molecular Dimensions), and 0.1M Morpheus Buffer System 2 (HEPES/MOPS buffer, Molecular Dimensions) pH 7.5.