5V5V
Complex of NLGN2 with MDGA1 Ig1-Ig2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2016-03-31 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 1 |
Unit cell lengths | 103.118, 97.182, 190.510 |
Unit cell angles | 95.52, 80.97, 88.71 |
Refinement procedure
Resolution | 49.770 - 4.110 |
R-factor | 0.29032 |
Rwork | 0.289 |
R-free | 0.31861 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bl8 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.550 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.770 | 4.180 |
High resolution limit [Å] | 4.110 | 4.110 |
Rmerge | 0.064 | 0.586 |
Number of reflections | 53553 | 2112 |
<I/σ(I)> | 18.8 | |
Completeness [%] | 94.4 | 76.1 |
Redundancy | 3.8 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.03 M NaNO3, 0.03 M Na2HPO4, 0.03 M (NH4)2SO4, 0.05 M HEPES pH 7.5, 0.05 M MOPS pH 7.5, 12 % (v/v) ethylene glycol, 9 % (w/v) PEG 8000 |