5V5U
Crystal structure of leucine-rich protein regulator, ElrR, from Enterococcus faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 108.712, 108.712, 144.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.617 - 2.278 |
| R-factor | 0.1977 |
| Rwork | 0.196 |
| R-free | 0.22730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.585 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.3.11) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.617 | 60.140 | 2.350 |
| High resolution limit [Å] | 2.280 | 9.110 | 2.280 |
| Rmerge | 0.063 | 0.028 | 1.564 |
| Rmeas | 0.067 | 0.031 | 1.675 |
| Rpim | 0.024 | 0.011 | 0.589 |
| Total number of observations | 321458 | 4853 | 27624 |
| Number of reflections | 40171 | ||
| <I/σ(I)> | 18.1 | 53.9 | 1.4 |
| Completeness [%] | 99.7 | 99 | 97.4 |
| Redundancy | 8 | 6.7 | 7.8 |
| CC(1/2) | 0.999 | 0.999 | 0.487 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291.15 | PEG 20000, PEG MME 550, NPS, MES/Imidazole |
