5V5T

Crystal structure of leucine-rich protein regulator, ElrR, from Enterococcus faecalis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-09-18
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1
Unit cell lengths	70.800, 75.330, 85.280
Unit cell angles	112.99, 89.93, 103.88

Refinement procedure

Resolution	36.550 - 2.150
R-factor	0.1992
Rwork	0.196
R-free	0.24160
Structure solution method	SAD, MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.140
Data reduction software	XDS
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.550	36.550	2.270
High resolution limit [Å]	2.150	6.800	2.150
Rmerge		0.024	0.542
Rmeas	0.062	0.031	0.705
Rpim	0.039	0.019	0.447
Total number of observations	185116	6345	17917
Number of reflections	76473
<I/σ(I)>	9.3	25.2	1.6
Completeness [%]	89.8	97.4	60.7
Redundancy	2.4	2.4	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291.15	PEG 1000, PEG 3350, MPD, MOPS/HEPES-Na, L-glutamate, DL-alanine, glycine, DL-lysine, DL-serine