5V5O
Structure of NLS2K of influenza A virus nucleoprotein bound to importin alpha
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.165, 90.893, 97.086 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.968 - 2.243 |
| R-factor | 0.1757 |
| Rwork | 0.174 |
| R-free | 0.20630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5huy |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.531 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2481)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rpim | 0.046 | 0.410 |
| Number of reflections | 32858 | |
| <I/σ(I)> | 29.5 | 3.5 |
| Completeness [%] | 97.8 | 96.3 |
| Redundancy | 3.9 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.1 M HEPES pH 6.0, 0.6 M sodium citrate tribasic dihydrate, 10 mM b-mercaptoethanol |
