5V5I

OTU protease of Crimean Congo Hemorrhagic Fever Virus bound to ubiquitin variant CC.1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-10-18
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 2 21 21
Unit cell lengths	40.167, 83.222, 150.886
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.167 - 2.201
R-factor	0.2259
Rwork	0.222
R-free	0.27270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.492
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((dev_2328: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.050	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.107	0.615
Number of reflections	26471
<I/σ(I)>	12.2	2.6
Completeness [%]	99.6	96.3
Redundancy	4.9	4.5
CC(1/2)	0.996	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	30% (w/v) PEG6000, 1.0M lithium chloride and 0.1M sodium acetate