5V5H
OTU protease of Crimean Congo Hemorrhagic Fever Virus bound to ubiquitin variant CC.2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-03 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.398, 33.599, 71.248 |
| Unit cell angles | 90.00, 96.51, 90.00 |
Refinement procedure
| Resolution | 43.434 - 1.500 |
| R-factor | 0.1575 |
| Rwork | 0.156 |
| R-free | 0.17810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pt2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.971 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2328: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.434 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.030 | 0.615 |
| Number of reflections | 38017 | 1876 |
| <I/σ(I)> | 18.7 | 2.1 |
| Completeness [%] | 98.7 | 96.6 |
| Redundancy | 3.7 | 3.6 |
| CC(1/2) | 0.999 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 30% (w/v) PEG 4000, 0.2 M CaCl2 and 0.1 M HEPES pH 7.5 |
