5V5F

Crystal structure of RICE1 (PNT2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	120
Detector technology	CCD
Collection date	2014-09-14
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	I 41 2 2
Unit cell lengths	193.250, 193.250, 214.719
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.963 - 2.945
R-factor	0.1697
Rwork	0.169
R-free	0.19460
RMSD bond length	0.008
RMSD bond angle	1.139
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.945
Number of reflections	42979
<I/σ(I)>	20.9
Completeness [%]	1.0
Redundancy	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	289	30% pentaerylthritol proxylate (5/4 PO/OH), 0.2 M KCl, 50 mM HEPES.