5V5C

VQIINK, Structure of the amyloid-spine from microtubule associated protein tau Repeat 2

Experimental procedure

Source type	ELECTRON MICROSCOPE
Source details	TECNAI F20 TEM
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-08-25
Detector	TVIPS TEMCAM-F416
Wavelength(s)	0.0251
Spacegroup name	P 21 21 2
Unit cell lengths	20.360, 43.220, 4.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.180 - 1.250
R-factor	0.2244
Rwork	0.219
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	10.180	10.180	1.310
High resolution limit [Å]	1.250	4.330	1.250
Rmerge	0.239	0.132	0.475
Rmeas	0.265	0.142	0.599
Total number of observations	5454
Number of reflections	1226	36	106
<I/σ(I)>	3.58	8.76	1.59
Completeness [%]	86.8	76.6	71.6
Redundancy	4.449	5.083	2.519
CC(1/2)	0.986	0.995	0.554

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	0.29 M lithium nitrate, 24% PEG3350