5V5C
VQIINK, Structure of the amyloid-spine from microtubule associated protein tau Repeat 2
Experimental procedure
Source type | ELECTRON MICROSCOPE |
Source details | TECNAI F20 TEM |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2016-08-25 |
Detector | TVIPS TEMCAM-F416 |
Wavelength(s) | 0.0251 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 20.360, 43.220, 4.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.180 - 1.250 |
R-factor | 0.2244 |
Rwork | 0.219 |
R-free | 0.26640 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 10.180 | 10.180 | 1.310 |
High resolution limit [Å] | 1.250 | 4.330 | 1.250 |
Rmerge | 0.239 | 0.132 | 0.475 |
Rmeas | 0.265 | 0.142 | 0.599 |
Total number of observations | 5454 | ||
Number of reflections | 1226 | 36 | 106 |
<I/σ(I)> | 3.58 | 8.76 | 1.59 |
Completeness [%] | 86.8 | 76.6 | 71.6 |
Redundancy | 4.449 | 5.083 | 2.519 |
CC(1/2) | 0.986 | 0.995 | 0.554 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 0.29 M lithium nitrate, 24% PEG3350 |