5V5B
KVQIINKKLD, Structure of the amyloid spine from microtubule associated protein tau Repeat 2
Experimental procedure
Source type | ELECTRON MICROSCOPE |
Source details | TECNAI F20 TEM |
Temperature [K] | 100 |
Detector technology | CMOS |
Collection date | 2016-08-25 |
Detector | TVIPS TEMCAM-F416 |
Wavelength(s) | 0.0251 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 27.120, 4.830, 32.430 |
Unit cell angles | 90.00, 100.53, 90.00 |
Refinement procedure
Resolution | 18.830 - 1.500 |
R-factor | 0.1927 |
Rwork | 0.190 |
R-free | 0.21270 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.510 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 18.830 | 18.830 | 1.560 |
High resolution limit [Å] | 1.500 | 5.410 | 1.500 |
Rmerge | 0.255 | 0.166 | 0.419 |
Rmeas | 0.268 | 0.177 | 0.452 |
Total number of observations | 20119 | ||
Number of reflections | 2203 | 28 | 281 |
<I/σ(I)> | 5.24 | 8.4 | 2.83 |
Completeness [%] | 84.8 | 62.2 | 85.9 |
Redundancy | 9.133 | 8.893 | 6.214 |
CC(1/2) | 0.984 | 0.979 | 0.849 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 310 | 0.1 M phosphate citrate, 32% PEG10000 |