5V4V
Saccharomyces cerevisiae Old Yellow Enzyme 3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2015-10-13 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9787 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.214, 107.762, 141.071 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.879 - 1.798 |
R-factor | 0.1496 |
Rwork | 0.147 |
R-free | 0.18910 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.245 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 38.880 |
High resolution limit [Å] | 1.798 |
Number of reflections | 87273 |
<I/σ(I)> | 20.2 |
Completeness [%] | 99.8 |
Redundancy | 14.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 50 mM MES monohydrate, 22% v/v PEG 400, 25 mM Tris, 25 mM NaCl, pH 8.0 |