5V4T
Protein 84 with aldehyde deformylating oxygenase activity from Sulfolobus tokodaii (orthorhombic form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-27 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979460 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 71.466, 114.360, 64.857 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.120 - 1.700 |
R-factor | 0.18978 |
Rwork | 0.189 |
R-free | 0.21963 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5uxg |
RMSD bond length | 0.017 |
RMSD bond angle | 1.726 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 38.120 |
High resolution limit [Å] | 1.700 |
Number of reflections | 59271 |
<I/σ(I)> | 18.5 |
Completeness [%] | 99.7 |
Redundancy | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 10% ethanol, 1.5 M NaCl |