5V4R

Crystal structure of LARP1-unique domain DM15 bound to m7GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-08-18
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	48.177, 60.163, 60.609
Unit cell angles	90.00, 101.28, 90.00

Refinement procedure

Resolution	33.895 - 1.770
R-factor	0.1787
Rwork	0.176
R-free	0.20940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4zc4
RMSD bond length	0.002
RMSD bond angle	0.481
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	33.895
High resolution limit [Å]	1.770
Number of reflections	28525
<I/σ(I)>	2.45
Completeness [%]	86.0
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES, pH 7.5 and 10% PEG 6000