5V4F
Crystal Structure of the Protein of Unknown Function of the Conserved Rid Protein Family YyfB from Yersinia pestis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 129.205, 129.205, 129.205 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.858 - 3.001 |
| R-factor | 0.1576 |
| Rwork | 0.155 |
| R-free | 0.19960 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.094 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((dev_2411)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.030 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 14594 | 740 |
| <I/σ(I)> | 10.1 | 2.03 |
| Completeness [%] | 99.3 | 100 |
| Redundancy | 4.8 | 4.8 |
| CC(1/2) | 0.669 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 298 | 0.1 M CHES pH 9.5, 30 5(w/v) PEG3000 |
