5V4A
A New Glycosyltransferase (DUF1792) from Streptococcus sanguinis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-16 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.00000 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 42.650, 99.860, 140.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.300 - 1.750 |
R-factor | 0.2086 |
Rwork | 0.206 |
R-free | 0.26850 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.943 |
Data scaling software | XDS |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 42.310 |
High resolution limit [Å] | 1.750 |
Number of reflections | 60928 |
<I/σ(I)> | 9.7 |
Completeness [%] | 94.3 |
Redundancy | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 2M Ammonium Sulfate, 2% PEG 400, 0.1M HEPES pH 7.5 |