5V45
Crystal structure of the F270M, K291M, L318M mutant of SR1 domain of human sacsin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-06-04 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.977 |
Spacegroup name | P 1 |
Unit cell lengths | 41.679, 41.360, 74.106 |
Unit cell angles | 99.55, 95.26, 107.50 |
Refinement procedure
Resolution | 50.000 - 1.910 |
R-factor | 0.18826 |
Rwork | 0.186 |
R-free | 0.22404 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.384 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 32566 | 2240 |
<I/σ(I)> | 43.5 | 4.5 |
Completeness [%] | 96.2 | 89.1 |
Redundancy | 7.9 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M MES pH 6.5, 28% PEG 3350, 0.2 M ammonium acetate |