5V44
Crystal structure of the SR1 domain of human sacsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.977 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.068, 41.721, 105.892 |
| Unit cell angles | 97.86, 95.76, 107.20 |
Refinement procedure
| Resolution | 50.000 - 1.560 |
| R-factor | 0.18362 |
| Rwork | 0.182 |
| R-free | 0.21445 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5v45 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.160 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.590 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Number of reflections | 86247 | 5846 |
| <I/σ(I)> | 34.6 | 2.9 |
| Completeness [%] | 96.3 | 87.96 |
| Redundancy | 4.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 28% PEG 3350, 0.2 M ammonium acetate, 0.1 M MES pH 6.5 |
