5V3N

Structure of S. cerevisiae Ulp2-Tof2-Csm1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	71.684, 56.933, 40.992
Unit cell angles	90.00, 121.06, 90.00

Refinement procedure

Resolution	35.115 - 1.300
R-factor	0.1934
Rwork	0.192
R-free	0.21710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3n4s
RMSD bond length	0.023
RMSD bond angle	1.735
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.000	1.320
High resolution limit [Å]	1.300	1.300
Rmerge	0.068	2.281
Rpim	0.042	1.418
Number of reflections	33373
<I/σ(I)>	10.1	1.1
Completeness [%]	96.4	92.7
Redundancy	3.7	3.5
CC(1/2)	0.995	0.418

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.2 M Ammonium Acetate, 0.1 M HEPES pH 7.5 and 25% PEG 3350 and 25% Glycerol