5V3M
mouseZFP568-ZnF1-11 in complex with DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-08-13 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 1.0000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 35.954, 92.758, 353.667 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.245 - 2.091 |
R-factor | 0.2148 |
Rwork | 0.213 |
R-free | 0.25320 |
Structure solution method | SAD |
RMSD bond length | 0.025 |
RMSD bond angle | 1.095 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.245 | 2.160 |
High resolution limit [Å] | 2.090 | 2.090 |
Rmerge | 0.067 | 0.479 |
Number of reflections | 53292 | 1160 |
<I/σ(I)> | 26 | 2.6 |
Completeness [%] | 81.7 | 32.9 |
Redundancy | 13.9 | 6.8 |
CC(1/2) | 0.910 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 289 | 10% Isopropanol, 0.1M Bicine(pH 8.5) and 30% (W/V) PEG1500 |