5V30
Crystal structure of the sensor domain of the transcriptional regulator HcpR from Porphyromonas Gingivalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-03-24 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 144.970, 144.970, 77.965 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.994 - 3.150 |
| R-factor | 0.1971 |
| Rwork | 0.195 |
| R-free | 0.23920 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.292 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.930 | 3.260 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.077 | 0.367 |
| Number of reflections | 14801 | 1429 |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 6.22 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.1M Na Acetate (pH 4.5), 1-2-1.4M Ammonium sulfate, 0.2M NaCl |
