5V2K

Crystal structure of UDP-glucosyltransferase, UGT74F2 (T15A), with UDP and 2-bromobenzoic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97972
Spacegroup name	P 21 21 21
Unit cell lengths	65.122, 87.270, 162.674
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.927 - 2.002
R-factor	0.1953
Rwork	0.193
R-free	0.24370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u6m
RMSD bond length	0.008
RMSD bond angle	0.918
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((dev_2666: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	63121
<I/σ(I)>	29.8
Completeness [%]	100.0
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	22-26 % (w/v) PEG 3350. 0.2 M ammonium acetate, 0.1 M MES