5V1Z
Crystal structure of the RPN13 PRU-RPN2 (932-953)-ubiquitin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.127 |
Spacegroup name | P 31 |
Unit cell lengths | 100.852, 100.852, 37.571 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.012 - 2.000 |
R-factor | 0.1552 |
Rwork | 0.146 |
R-free | 0.18170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 2R2Y & 1CMX |
RMSD bond length | 0.009 |
RMSD bond angle | 0.887 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 28875 | 2748 |
<I/σ(I)> | 16 | 2.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 4.7 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.1 M sodium acetate, pH 4.6, 22.5% PEG3350 |