5V1Y
Crystal structure of the ternary RPN13 PRU-RPN2 (940-953)-ubiquitin complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.075 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.129, 96.098, 57.605 |
Unit cell angles | 90.00, 96.38, 90.00 |
Refinement procedure
Resolution | 36.804 - 1.421 |
R-factor | 0.1413 |
Rwork | 0.140 |
R-free | 0.17480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 2R2Y & 1CMX |
RMSD bond length | 0.008 |
RMSD bond angle | 0.984 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.470 |
High resolution limit [Å] | 1.420 | 1.420 |
Number of reflections | 72405 | 2526 |
<I/σ(I)> | 24.8 | 5.2 |
Completeness [%] | 98.0 | 93 |
Redundancy | 7.6 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 277 | 0.1 M citric acid, pH 4.6, 20% PEG6000 |