5V1X
Carbon Sulfoxide lyase, Egt2 Y134F in complex with its substrate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 103.898, 195.067, 107.604 |
| Unit cell angles | 90.00, 91.34, 90.00 |
Refinement procedure
| Resolution | 48.806 - 2.558 |
| R-factor | 0.2234 |
| Rwork | 0.223 |
| R-free | 0.27930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uts |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.632 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.558 |
| Number of reflections | 135254 |
| <I/σ(I)> | 10.69 |
| Completeness [%] | 99.4 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 30% PEG 3350, 50mM HEPES pH 7.0, 0.2M sodium formate |
