5V1V
TbiB1 in Complex with the TbiA(alpha) Leader Peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-07 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.978720 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.109, 60.227, 62.451 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.350 |
R-factor | 0.19391 |
Rwork | 0.192 |
R-free | 0.22684 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.060 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.100 | 1.355 |
High resolution limit [Å] | 1.350 | 1.351 |
Number of reflections | 40418 | 409 |
<I/σ(I)> | 19.7 | 2.3 |
Completeness [%] | 99.8 | 95.1 |
Redundancy | 6.8 | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 282 | 8% isopropanol, 0.3 M zinc acetate, 0.1 M sodium cacodylate (pH 6.5), 10 mg/mL Protein, 1 mM TbiAalpha Leader Peptide |