5V1U
TbiB1 in Complex with the TbiA(beta) Leader Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-08 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.978560 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.155, 48.630, 55.287 |
| Unit cell angles | 78.30, 66.88, 88.12 |
Refinement procedure
| Resolution | 31.257 - 2.052 |
| R-factor | 0.2025 |
| Rwork | 0.201 |
| R-free | 0.23990 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.853 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 23055 | 1003 |
| <I/σ(I)> | 17.6 | 2.9 |
| Completeness [%] | 91.2 | 82.6 |
| Redundancy | 2.7 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | 4% isopropanol, 0.05 M zinc acetate, 0.1 M sodium cacodylate (pH 6.5), 6 mg/mL Protein, 1 mM TbiA(beta) T(-5)E |
