5V1R

DNA polymerase beta reactant complex with 8-oxoG:C at the primer terminus and incoming dCTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-15
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	50.596, 79.730, 55.647
Unit cell angles	90.00, 107.53, 90.00

Refinement procedure

Resolution	24.977 - 2.077
R-factor	0.1844
Rwork	0.180
R-free	0.23690
RMSD bond length	0.007
RMSD bond angle	0.978
Data scaling software	HKL-3000
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.077
Number of reflections	44071
<I/σ(I)>	17.3
Completeness [%]	99.4
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	50 mM imidazole, 350 mM sodium acetate, 17% PEG3350