5V1Q
Crystal structure of Streptococcus suis SuiB
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-19 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 115.230, 84.424, 110.044 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.509 - 2.500 |
R-factor | 0.2184 |
Rwork | 0.217 |
R-free | 0.25680 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.659 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.510 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.096 | 1.290 |
Number of reflections | 37704 | |
<I/σ(I)> | 17.7 | 1.9 |
Completeness [%] | 99.3 | 94.4 |
Redundancy | 12.9 | 11.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 285 | A solution containing 18.9 mg/mL His6-tagged SuiB in storage buffer [100 mM HEPES, pH 7.5, 300 mM KCl, 5 mM DTT, 10% (v/v) glycerol] was mixed 1:1 with precipitant solution. Small clusters of crystals were formed within 24 hrs. A seed stock was then produced by combining a single sitting well with 10 uL of reservoir solution and 10 uL of enzyme at 8 mg/mL, followed by brief vortexing. A resulting crystal was harvested 2 days following seeding. The precipitant solution was 100 mM MES, pH 6.0, 15% (w/v) PEG 3350. |