5V1K
Structure of R-GNA dodecamer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2013-02-14 |
Detector | MARRESEARCH |
Wavelength(s) | 0.916978 |
Spacegroup name | P 1 |
Unit cell lengths | 23.681, 27.680, 29.050 |
Unit cell angles | 73.00, 87.17, 66.58 |
Refinement procedure
Resolution | 50.000 - 1.180 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXDE |
Refinement software | SHELXL |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.220 |
High resolution limit [Å] | 1.180 | 1.180 |
Rmerge | 0.052 | 0.449 |
Number of reflections | 20171 | 1914 |
<I/σ(I)> | 37.5 | |
Completeness [%] | 94.9 | 90.3 |
Redundancy | 4.3 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | Oligonucleotide (0.6 mM), sodium cacodylate (20 mM, pH 6.0), potassium chloride (40 mM), spermine tetrahydrochloride (6 mM), and 2-methyl-2,4-pentanediol (MPD; 5% (v/v) |