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5V1K

Structure of R-GNA dodecamer

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 21-ID-D
Synchrotron siteAPS
Beamline21-ID-D
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date2013-02-14
DetectorMARRESEARCH
Wavelength(s)0.916978
Spacegroup nameP 1
Unit cell lengths23.681, 27.680, 29.050
Unit cell angles73.00, 87.17, 66.58
Refinement procedure
Resolution50.000 - 1.180
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareSHELXDE
Refinement softwareSHELXL
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0001.220
High resolution limit [Å]1.1801.180
Rmerge0.0520.449
Number of reflections201711914
<I/σ(I)>37.5
Completeness [%]94.990.3
Redundancy4.34
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP6291Oligonucleotide (0.6 mM), sodium cacodylate (20 mM, pH 6.0), potassium chloride (40 mM), spermine tetrahydrochloride (6 mM), and 2-methyl-2,4-pentanediol (MPD; 5% (v/v)

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