5V1G
DNA polymerase beta binary complex with 8-oxoG at the primer terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-29 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.380, 79.240, 54.880 |
| Unit cell angles | 90.00, 105.49, 90.00 |
Refinement procedure
| Resolution | 24.878 - 1.798 |
| R-factor | 0.1909 |
| Rwork | 0.187 |
| R-free | 0.22720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3isb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.903 |
| Data scaling software | HKL-3000 |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.798 |
| Number of reflections | 69873 |
| <I/σ(I)> | 20.6 |
| Completeness [%] | 98.3 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 50 mM imidazole, 350 mM sodium acetate, 17% PEG3350 |
