5V1A

Structure of S. cerevisiae Ulp2:Csm1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-01
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.18
Spacegroup name	P 43 21 2
Unit cell lengths	46.737, 46.737, 124.638
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.761 - 2.140
R-factor	0.2391
Rwork	0.234
R-free	0.27820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3n4s
RMSD bond length	0.003
RMSD bond angle	0.565
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.000	2.200
High resolution limit [Å]	2.140	2.140
Rpim		0.367
Number of reflections	8129
<I/σ(I)>	53.9
Completeness [%]	98.7
Redundancy	13.8
CC(1/2)		0.699

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	100 mM M HEPES pH 7.5 and 20% PEG 3350, 25% Glycerol