5V0M

SeMet crystal structure of the Neisseria meningitidis non-core minor pilin PilV in the monoclinic form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.96859
Spacegroup name	C 1 2 1
Unit cell lengths	46.320, 21.800, 83.830
Unit cell angles	90.00, 104.24, 90.00

Refinement procedure

Resolution	27.084 - 1.407
R-factor	0.1741
Rwork	0.172
R-free	0.20640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5v23
RMSD bond length	0.009
RMSD bond angle	1.024
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.630	1.430
High resolution limit [Å]	1.407	1.410
Rmerge	0.098	0.819
Rpim	0.043	0.359
Number of reflections	15424
<I/σ(I)>	12.2	4
Completeness [%]	95.9	97
Redundancy	5.9	6
CC(1/2)	0.997	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	100 mM Sodium Acetate pH 5, 100 mM ammonium chloride, 28 % w/v PEG 8000