5V0J
RNA duplex with 2-MeImpG analogue bound-2 binding sites
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 99 |
| Detector technology | CCD |
| Collection date | 2016-04-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 43.388, 43.388, 253.851 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 84.620 - 1.500 |
| R-factor | 0.23892 |
| Rwork | 0.237 |
| R-free | 0.27019 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hbx |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.963 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.041 | 0.575 |
| Number of reflections | 15290 | 744 |
| <I/σ(I)> | 35.37 | 1.84 |
| Completeness [%] | 99.4 | 99.9 |
| Redundancy | 6.3 | 5.6 |
| CC(1/2) | 0.804 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 10% MPD, 0.040 M sodium cacodylate pH7.0, 0.012 M spermine tetrahydrochloride, 0.08 M sodium chloride, 0.02 M magnesium chloride |
