5V0B

Crystal structure of human exonuclease 1 Exo1 (WT) in complex with 5' recessed-end DNA (rIX)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	73.764, 73.764, 182.796
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.850 - 2.630
R-factor	0.211
Rwork	0.207
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qea
RMSD bond length	0.003
RMSD bond angle	0.544
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (DEV_1839)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.680
High resolution limit [Å]	2.630	2.630
Rmerge	0.099	0.674
Rmeas	0.108	0.740
Number of reflections	15270	763
<I/σ(I)>	18.09	3.09
Completeness [%]	96.6	99.5
Redundancy	5.9	5.7
CC(1/2)	0.793

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	290	100 mM sodium acetate, pH 7.0, 10 mM potassium chloride, 2-4% PEG4000