5V07

Crystal structure of human exonuclease 1 Exo1 (D173A) in complex with 5' recessed-end DNA (rV)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-22
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1
Spacegroup name	P 43 21 2
Unit cell lengths	73.837, 73.837, 179.067
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.919 - 2.150
R-factor	0.1795
Rwork	0.176
R-free	0.21970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3qea
RMSD bond length	0.012
RMSD bond angle	1.036
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.190
High resolution limit [Å]	2.150	2.150
Rmerge	0.084	0.308
Rmeas	0.090	0.380
Number of reflections	22902	355
<I/σ(I)>	23.7	2.83
Completeness [%]	80.3	25.5
Redundancy	7.2	2.2
CC(1/2)	0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	290	100 mM sodium acetate, pH 7.0, 10 mM potassium chloride, 2-4% PEG4000