5UZQ
Crystal structure of citrate synthase from homo sapiens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 76.760, 76.760, 198.764 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.160 |
| R-factor | 0.19789 |
| Rwork | 0.196 |
| R-free | 0.23232 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.474 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.190 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.068 | 0.323 |
| Rmeas | 0.094 | 0.483 |
| Rpim | 0.029 | 0.148 |
| Number of reflections | 29021 | 1500 |
| <I/σ(I)> | 25.87 | 3.29 |
| Completeness [%] | 93.0 | 93.6 |
| Redundancy | 8.5 | 8.5 |
| CC(1/2) | 0.968 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | 0.1 M Succinic Acid pH 7.0, 12% PEG3350 |
