5UZG
Crystal structure of Glorund qRRM1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-28 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 115.240, 27.720, 66.415 |
| Unit cell angles | 90.00, 109.39, 90.00 |
Refinement procedure
| Resolution | 23.984 - 1.541 |
| R-factor | 0.1756 |
| Rwork | 0.174 |
| R-free | 0.20850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2kfy |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.038 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.540 |
| Rmerge | 0.065 |
| Number of reflections | 26983 |
| <I/σ(I)> | 20.2 |
| Completeness [%] | 90.9 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2 M ammonium sulfate, 0.1 M Na acetate pH 4.6, 30% w/v PEGMME 2000 |
