5UZG
Crystal structure of Glorund qRRM1 domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-28 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 115.240, 27.720, 66.415 |
Unit cell angles | 90.00, 109.39, 90.00 |
Refinement procedure
Resolution | 23.984 - 1.541 |
R-factor | 0.1756 |
Rwork | 0.174 |
R-free | 0.20850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2kfy |
RMSD bond length | 0.009 |
RMSD bond angle | 1.038 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.540 |
Rmerge | 0.065 |
Number of reflections | 26983 |
<I/σ(I)> | 20.2 |
Completeness [%] | 90.9 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.2 M ammonium sulfate, 0.1 M Na acetate pH 4.6, 30% w/v PEGMME 2000 |