5UYV
YfeA ancillary sites that do not co-load with site 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.27932 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.516, 51.959, 112.751 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.959 - 1.691 |
R-factor | 0.1755 |
Rwork | 0.173 |
R-free | 0.20610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5uxs |
RMSD bond length | 0.007 |
RMSD bond angle | 0.841 |
Data reduction software | HKL-2000 (v712) |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.720 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmerge | 0.082 | 0.259 |
Rpim | 0.052 | 0.186 |
Number of reflections | 27201 | 1217 |
<I/σ(I)> | 43.7 | 4.1 |
Completeness [%] | 96.8 | 88.4 |
Redundancy | 3.2 | 2.7 |
CC(1/2) | 0.948 | 0.752 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.3 | 293.15 | 30% PEG 4000, 20mM Bis-tris propane, 50mM sodium chloride, 0.05% w/v sodium azide SOAKING: Fiber used to streak 1.5uL droplet of 10mM zinc chloride into crystallization drop. Mixture incubated 4-hrs prior to freezing. |