5UYR
Crystal structure of the dark-adapted full-length bacteriophytochrome XccBphP mutant D199A from Xanthomonas campestris
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-11 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9801 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 103.860, 103.860, 342.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.270 - 3.450 |
R-factor | 0.231 |
Rwork | 0.230 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5akp |
RMSD bond length | 0.010 |
RMSD bond angle | 1.190 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.940 | 3.690 |
High resolution limit [Å] | 3.450 | 3.450 |
Rmerge | 0.165 | 1.536 |
Rpim | 0.048 | 0.459 |
Number of reflections | 25772 | 4569 |
<I/σ(I)> | 10.6 | 1.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 12.4 | 12 |
CC(1/2) | 0.990 | 0.690 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 293 | 7%(W/V) PEG 4000 0.2 M SODIUM ACETATE 0.1 M TRIS |