5UYJ

Crystal Structure of the Human CAMKK2B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.976250
Spacegroup name	P 43 21 2
Unit cell lengths	73.153, 73.153, 120.305
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.610 - 1.600
R-factor	0.154
Rwork	0.153
R-free	0.17100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zv2
RMSD bond length	0.010
RMSD bond angle	1.040
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.610	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.122	0.315
Rpim	0.025	0.063
Number of reflections	43864	2137
<I/σ(I)>	21.3	10.9
Completeness [%]	99.9	100
Redundancy	25.8
CC(1/2)	0.997	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	20% PEG 3350, 20 mM Ammonium Sulphate, 0.1M Citric acid-Hepes-CHES buffer system