5UYB
YfeA from M9 minimal media supplemented with manganese - Fe wavelength
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-01-10 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 1.74013 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.746, 52.186, 112.744 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.149 - 1.950 |
R-factor | 0.1688 |
Rwork | 0.165 |
R-free | 0.20170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5uya |
RMSD bond length | 0.006 |
RMSD bond angle | 0.764 |
Data reduction software | HKL-2000 (v712) |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.092 | 0.402 |
Rpim | 0.035 | 0.158 |
Number of reflections | 18617 | 921 |
<I/σ(I)> | 52.7 | 5.2 |
Completeness [%] | 99.7 | 99.9 |
Redundancy | 7.6 | 7.5 |
CC(1/2) | 0.995 | 0.941 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.3 | 293.15 | 30% PEG 4000, 20mM Bis-tris propane, 50mM sodium chloride, 0.005% w/v sodium azide |