5UY6
Crystal Structure of the Human CAMKK2B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-09 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.979200 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 73.324, 73.324, 121.964 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.750 - 1.700 |
R-factor | 0.161 |
Rwork | 0.160 |
R-free | 0.18200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zv2 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.050 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.750 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.147 | 1.837 |
Rpim | 0.032 | 0.398 |
Number of reflections | 37374 | 1939 |
<I/σ(I)> | 14.2 | 2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 21.7 | 22.1 |
CC(1/2) | 0.998 | 0.751 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 25% PEG 3350; 0.1M Bis-Tris; 0.2M ammonium acetate |