5UXX
Co-crystal structure of the sigma factor RpoE in complex with the anti-sigma factor NepR from Bartonella quintana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-07-16 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 92.420, 92.420, 127.740 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.210 - 2.450 |
R-factor | 0.192 |
Rwork | 0.191 |
R-free | 0.22300 |
Structure solution method | SAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.523 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | RESOLVE |
Refinement software | PHENIX (dev_1738) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.210 | 2.510 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.106 | 0.516 |
Number of reflections | 44774 | |
<I/σ(I)> | 10.75 | 2.71 |
Completeness [%] | 99.7 | 99.6 |
Redundancy | 3.75 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 289 | BaquA.17208.a.CB1.PS01985 at 24.97mg/ml was mixed 1:1 with a condition based on MCSG2(b11): 29% (w/v) PEG-3350, 0.2 M lithium sulfate, 0.1 M Bis-Tris/HCl, pH = 7.2. |