5UXT

Coiled-coil Trimer with Glu:Trp:Lys Triad

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-09-23
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5
Spacegroup name	C 1 2 1
Unit cell lengths	84.815, 38.516, 37.031
Unit cell angles	90.00, 113.34, 90.00

Refinement procedure

Resolution	22.342 - 2.197
R-factor	0.2024
Rwork	0.194
R-free	0.27450
RMSD bond length	0.007
RMSD bond angle	0.838
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.280
High resolution limit [Å]	2.197	4.740	2.200
Rmerge	0.133	0.079	0.393
Rmeas	0.153	0.091	0.477
Rpim	0.073	0.043	0.265
Number of reflections	5259
<I/σ(I)>	7.8
Completeness [%]	92.0	99.2	62.7
Redundancy	3.9	4.4	2.3
CC(1/2)		0.997	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	0.3 uL protein + 0.3 uL 50% v/v PEG200, 0.1 M sodium/potassium phosphate, pH 6.2, 0.2 M sodium chloride