5UXT
Coiled-coil Trimer with Glu:Trp:Lys Triad
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2016-09-23 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 84.815, 38.516, 37.031 |
Unit cell angles | 90.00, 113.34, 90.00 |
Refinement procedure
Resolution | 22.342 - 2.197 |
R-factor | 0.2024 |
Rwork | 0.194 |
R-free | 0.27450 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.838 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 2.280 |
High resolution limit [Å] | 2.197 | 4.740 | 2.200 |
Rmerge | 0.133 | 0.079 | 0.393 |
Rmeas | 0.153 | 0.091 | 0.477 |
Rpim | 0.073 | 0.043 | 0.265 |
Number of reflections | 5259 | ||
<I/σ(I)> | 7.8 | ||
Completeness [%] | 92.0 | 99.2 | 62.7 |
Redundancy | 3.9 | 4.4 | 2.3 |
CC(1/2) | 0.997 | 0.822 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 0.3 uL protein + 0.3 uL 50% v/v PEG200, 0.1 M sodium/potassium phosphate, pH 6.2, 0.2 M sodium chloride |