5UXQ
Structure of anti-HIV trimer apex antibody PGT143
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-05 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.033 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.866, 97.643, 126.206 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.822 - 2.415 |
R-factor | 0.2314 |
Rwork | 0.229 |
R-free | 0.26680 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.900 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.830 |
High resolution limit [Å] | 2.400 |
Number of reflections | 32477 |
<I/σ(I)> | 9.6 |
Completeness [%] | 95.1 |
Redundancy | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 295 | 65% (v/v) 2-methyl-2,4-pentanediol and 0.1 M Tris, pH 8.0 |