5UXM
Type II DAH7PS from Pseudomonas aeruginosa with Trp bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2016-02-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 81.680, 100.370, 115.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.790 - 1.540 |
| R-factor | 0.1861 |
| Rwork | 0.185 |
| R-free | 0.21039 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b7o |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.432 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.790 | 1.570 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.106 | 1.122 |
| Rpim | 0.044 | 0.452 |
| Number of reflections | 70122 | 3437 |
| <I/σ(I)> | 9.3 | 1.5 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 7 | 7.2 |
| CC(1/2) | 0.995 | 0.702 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 278 | 0.1 M sodium acetate pH 5.5, 0.8 M Sodium formate, 1 mM cobalt chloride, 1 mM phosphoenol pyruvate 26 % PEG 2000 MME |
