5UXA
Crystal structure of macrolide 2'-phosphotransferase MphB from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 67.276, 116.684, 92.574 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.171 - 1.950 |
| R-factor | 0.155 |
| Rwork | 0.151 |
| R-free | 0.20130 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.156 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_2481) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rpim | 0.033 | 0.282 |
| Number of reflections | 27167 | 1354 |
| <I/σ(I)> | 24.94 | 3.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.6 | 8.7 |
| CC(1/2) | 0.905 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.2 M calcium acetate, 0.1 M sodium cacodylate pH 6.5, 18% PEG 8K |
