5UX2

Protein 19 with aldehyde deformylating oxidase activity from Synechococcus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-13
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	50.986, 90.227, 109.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	109.060 - 1.990
R-factor	0.21238
Rwork	0.211
R-free	0.25355
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uw2
RMSD bond length	0.011
RMSD bond angle	1.355
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	109.060
High resolution limit [Å]	1.990
Rmerge	0.057
Number of reflections	33222
<I/σ(I)>	11.9
Completeness [%]	95.3
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	25.9 mg/ml 17% PEG 4000, 8.5 isopropanol, 85 mM Hepes