5UX2
Protein 19 with aldehyde deformylating oxidase activity from Synechococcus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-13 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.986, 90.227, 109.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 109.060 - 1.990 |
R-factor | 0.21238 |
Rwork | 0.211 |
R-free | 0.25355 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5uw2 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.355 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 109.060 |
High resolution limit [Å] | 1.990 |
Rmerge | 0.057 |
Number of reflections | 33222 |
<I/σ(I)> | 11.9 |
Completeness [%] | 95.3 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 25.9 mg/ml 17% PEG 4000, 8.5 isopropanol, 85 mM Hepes |