5UX1

Protein 43 with aldehyde deformylating oxygenase activity from Synechococcus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.12710
Spacegroup name	P 1 21 1
Unit cell lengths	72.342, 78.274, 83.734
Unit cell angles	90.00, 103.20, 90.00

Refinement procedure

Resolution	81.500 - 1.500
R-factor	0.19226
Rwork	0.192
R-free	0.21473
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.396
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.500	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.035	0.562
Number of reflections	143854
<I/σ(I)>	19.5	2.3
Completeness [%]	99.4	95.8
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	14% PEG 3350, 140 mM ammonium formate