5UX1
Protein 43 with aldehyde deformylating oxygenase activity from Synechococcus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-05-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.12710 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.342, 78.274, 83.734 |
Unit cell angles | 90.00, 103.20, 90.00 |
Refinement procedure
Resolution | 81.500 - 1.500 |
R-factor | 0.19226 |
Rwork | 0.192 |
R-free | 0.21473 |
Structure solution method | MAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.396 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.500 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.035 | 0.562 |
Number of reflections | 143854 | |
<I/σ(I)> | 19.5 | 2.3 |
Completeness [%] | 99.4 | 95.8 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 14% PEG 3350, 140 mM ammonium formate |